A stability indicating RP-HPLC method has been developed and validated for quantitative determination of Lamivudine, Emtricitabine, Tenofovir Disoproxil Fumarate and Efavirenz in different Pharmaceutical dosage forms. Chromatographic separation was achieved through gradient elution. Detection wavelength was monitored at 260 nm. The retention times of the Lamivudine, Emtricitabine, Tenofovir disoproxil fumarate and Efavirenz was about 3.2, 5.97, 8.15 and 12.52 min respectively. The developed method was validated as per ICH guidelines. This method is found to be simple, fast and economical. Hence this validated method can be used in routine quality testing of individual dosage forms and combination dosage forms of Lamivudine, Emtricitabine, Tenofovir disoproxil fumarate and Efavirenz.
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